Impaired replication of hepatitis C virus containing mutations in a conserved NS5B retinoblastoma protein-binding motif.

Impaired Replication of Hepatitis C Virus Containing Mutations in a Conserved NS 5 B 3 Retinoblastoma Protein - Binding Motif

23 HCV down-regulates the retinoblastoma tumor suppressor protein (Rb), a central cell 24 cycle regulator which is also targeted by oncoproteins expressed by DNA tumor viruses. HCV 25 genome replication is also enhanced in proliferating cells. Thus, it is possible that HCV 26 interactions with host cell cycle regulators, such as Rb, have evolved to modify the intracellular 27 environment to pro...

Mutations in the retinoblastoma protein-binding LXCXE motif of rubella virus putative replicase affect virus replication.

The rubella virus (RV)-encoded protein NSP90, which contains the retinoblastoma protein (Rb)-binding motif LXCXE, interacts with Rb and RV replication is reduced in cells lacking Rb. Whether the LXCXE motif of RV NSP90 itself is essential for Rb binding and virus replication is not known. Therefore, in the present study, the functional role of this motif was investigated by site-directed mutage...

Detection of Pre-treatment mutations leading to resistance to direct hepatitis C virus blocking drugs in patients with chronic hepatitis C

Background and objective: Human is the only host of hepatitis C virus. This virus has a positive single stranded RNA and lipoprotein envelop that has 7 confirmed genotypes. According to studies, genotypes 1a, 3a and 1b are the most common genotypes in Iran. No effective vaccine against HCV infection has been developed instead, advances in antiviral treatment using drugs that directly affect spe...

The C-terminal hydrophobic domain of hepatitis C virus RNA polymerase NS5B can be replaced with a heterologous domain of poliovirus protein 3A.

Replication of the plus-stranded RNA genome of hepatitis C virus (HCV) occurs in a membrane-bound replication complex consisting of viral and cellular proteins and viral RNA. NS5B, the RNA polymerase of HCV, is anchored to the membranes via a C-terminal 20-amino-acid-long hydrophobic domain, which is flanked on each side by a highly conserved positively charged arginine. Using a genotype 1b sub...

CpG Motif as an Adjuvant in Immunization of a Recombinant Plasmid Encoding Hepatitis C Virus Core Protein